Signalling

Part:BBa_K182200:Design

Designed by: Jennifer Kleine-Albers and Katriona McMichael   Group: iGEM09_Aberdeen_Scotland   (2009-07-07)


Constitutively expressed LuxR/LuxI operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 701
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoter J23107 was chosen because other promoters are available with a range of different strengths, and in addition functional quorum sensing was reported by the Heidelberg Team, 2008, using this promoter.


Source

All parts sourced from existing Biobricks.

References